Characterization of the 3a protein of SARS-associated coronavirus in infected vero E6 cells and SARS patients.

نویسندگان

  • Rong Zeng
  • Rui-Fu Yang
  • Mu-De Shi
  • Man-Rong Jiang
  • You-Hua Xie
  • Hong-Qiang Ruan
  • Xiao-Sheng Jiang
  • Lv Shi
  • Hu Zhou
  • Lei Zhang
  • Xiao-Dong Wu
  • Ying Lin
  • Yong-Yong Ji
  • Lei Xiong
  • Yan Jin
  • Er-Hei Dai
  • Xiao-Yi Wang
  • Bin-Ying Si
  • Jin Wang
  • Hong-Xia Wang
  • Cui-E Wang
  • Yong-Hua Gan
  • Yu-Chuan Li
  • Ju-Tian Cao
  • Jiang-Ping Zuo
  • Shi-Fang Shan
  • En Xie
  • Song-Hua Chen
  • Zhi-Qin Jiang
  • Xi Zhang
  • Yuan Wang
  • Gang Pei
  • Bing Sun
  • Jia-Rui Wu
چکیده

Proteomics was used to identify a protein encoded by ORF 3a in a SARS-associated coronavirus (SARS-CoV). Immuno-blotting revealed that interchain disulfide bonds might be formed between this protein and the spike protein. ELISA indicated that sera from SARS patients have significant positive reactions with synthesized peptides derived from the 3a protein. These results are concordant with that of a spike protein-derived peptide. A tendency exists for co-mutation between the 3a protein and the spike protein of SARS-CoV isolates, suggesting that the function of the 3a protein correlates with the spike protein. Taken together, the 3a protein might be tightly correlated to the spike protein in the SARS-CoV functions. The 3a protein may serve as a new clinical marker or drug target for SARS treatment.

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عنوان ژورنال:
  • Journal of molecular biology

دوره 341 1  شماره 

صفحات  -

تاریخ انتشار 2004